![]() ![]() Click Workspace>Serial Section Manager to define sections.Select the lens that matches the objective from the drop-down menu in the toolbar.41, we know that you can obtain a volume estimate with a 10-15% coefficient of error from 10-15 evenly spaced systematic random sections through the region of interest, and a grid spacing that results in ~200 marker points in total (not per section).įor volume estimation, you also need to know either the thickness of the sections to be sampled, or the thickness interval between the sections to be sampled. The more points you have, the more decisions you have to make.īased on theory and practical experience Howard and Reed, Unbiased Stereology, First Edition, p. ![]() It is up to you to find the right balance between precision and efficiency. Larger grid spacing saves time, but may result in reduced precision.Smaller grid spacing results in a more precise estimate, but requires placing more markers.The grid spacing determines the number of markers placed. You need to determine an appropriate distance between the points of the point grid that will be overlaid on the region of interest. Because it is not possible to draw a dimensionless point, we represent this concept as a cross or plus sign, where a unique feature of the cross (such as the lower left quadrant of the cross) is used to consistently mark the points which overlay the region of interest. With a grid, the intersections of each of the grid lines are related to the areas formed by the grid.īy associating one area with a point (more accurately, a dimensionless point) located at each intersection, the entire area of the grid is represented.Įach point represents a given area. The Cavalieri Estimator is a grid of dimensionless points randomly placed on the region of interest to be estimated. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |